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HIST1H2BC (Ab-34) Antibody (PACO60482)

SKU:
PACO60482
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
IF
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
€349
Frequently bought together:

Description

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HIST1H2BC (Ab-34) Antibody (PACO60482)

The Hist1H2bc (AB-34) Antibody (PAC060482) is a polyclonal antibody designed for research involving Hist1H2bc, a histone protein that plays a crucial role in chromatin structure and gene regulation. The antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in immunohistochemistry and immunofluorescence applications. It specifically targets the Hist1H2bc protein, allowing for accurate detection and analysis in various cellular contexts.Hist1H2bc is a member of the histone H2B family, which functions in packaging DNA into nucleosomes and regulating gene expression.

Alterations in histone proteins like Hist1H2bc have been linked to various diseases, including cancer and neurological disorders. Studying the function and regulation of Hist1H2bc can provide insights into these disease mechanisms and potential therapeutic targets.The Hist1H2bc (AB-34) Antibody is a valuable tool for researchers investigating chromatin structure, epigenetics, and gene regulation. Its specificity and high reactivity make it ideal for experiments aimed at understanding the role of Hist1H2bc in cellular processes and disease pathogenesis.

Antibody Name:HIST1H2BC (Ab-34) Antibody (PACO60482)
Antibody SKU:PACO60482
Size:50ul
Host Species:Rabbit
Tested Applications:ELISA, IF
Recommended Dilutions:ELISA:1:2000-1:10000, IF:1:1-1:10
Species Reactivity:Human
Immunogen:Peptide sequence around site of Lys (34) derived from Human Histone H2B type 1-C/E/F/G/I
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:Antigen Affinity Purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageImmunofluorescence staining of Hela cells with PACO60482 at 1:1, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Synonyms:Histone H2B type 1-C/E/F/G/I (Histone H2B.1 A) (Histone H2B.a) (H2B/a) (Histone H2B.g) (H2B/g) (Histone H2B.h) (H2B/h) (Histone H2B.k) (H2B/k) (Histone H2B.l) (H2B/l), HIST1H2BC; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BI, H2BFL; H2BFH; H2BFG; H2BFA; H2BFK
UniProt Protein Function:H2B1C: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 6p22.1

Cellular Component: cytoplasm; extracellular space; nucleoplasm; nucleus

Molecular Function:DNA binding; protein binding

Biological Process: antibacterial humoral response; defense response to Gram-positive bacterium; innate immune response in mucosa; nucleosome assembly

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein has antibacterial and antifungal antimicrobial activity. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
UniProt Code:P62807
NCBI GenInfo Identifier:290457686
NCBI Gene ID:8339
NCBI Accession:P62807.4
UniProt Secondary Accession:P62807,P02278, Q3B872, Q4VB69, Q93078, Q93080,
UniProt Related Accession:P62807
Molecular Weight:13,906 Da
NCBI Full Name:Histone H2B type 1-C/E/F/G/I
NCBI Synonym Full Names:histone cluster 1, H2bg
NCBI Official Symbol:HIST1H2BG  
NCBI Official Synonym Symbols:H2B/a; H2BFA; H2B.1A; dJ221C16.8  
NCBI Protein Information:histone H2B type 1-C/E/F/G/I
UniProt Protein Name:Histone H2B type 1-C/E/F/G/I
UniProt Synonym Protein Names:Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l
Protein Family:Histone
UniProt Gene Name:HIST1H2BC  
UniProt Entry Name:H2B1C_HUMAN
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