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HIST1H2BC (Ab-120) Antibody (PACO60478)

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SKU:
PACO60478
Product Type:
Antibody
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
WB
ChIP
Modification Type:
Histone
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
  • HIST1H2BC Ab-120 Antibody PACO60478
  • HIST1H2BC Ab-120 Antibody PACO60478
€349

Description

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HIST1H2BC (Ab-120) Antibody (PACO60478)

The Hist1H2bc Polyclonal Antibody (PACO60478) is a valuable tool for researchers studying histone proteins and their role in gene regulation and chromatin structure. Histone proteins, including Hist1H2bc, play a critical role in packaging DNA into chromatin and regulating gene expression.This antibody, raised in rabbits, specifically targets the Hist1H2bc protein and is highly reactive with human samples. It is validated for use in Western blot applications, enabling researchers to detect and analyze Hist1H2bc in various cell types.

This makes it an ideal tool for studies in epigenetics, cancer research, and developmental biology.Understanding the function and regulation of histone proteins like Hist1H2bc is essential for unraveling the complex mechanisms of gene expression and epigenetic modifications. By targeting Hist1H2bc with this antibody, researchers can further explore the role of histones in health and disease, paving the way for new insights and potential therapeutic interventions.

Antibody Name:HIST1H2BC (Ab-120) Antibody (PACO60478)
Antibody SKU:PACO60478
Size:50ul
Host Species:Rabbit
Tested Applications:ELISA, WB, ChIP
Recommended Dilutions:ELISA:1:2000-1:10000, WB:1:100-1:1000
Species Reactivity:Human, Rat
Immunogen:Peptide sequence around site of Lys (120) derived from Human Histone H2B type 1-C/E/F/G/I
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:Antigen Affinity Purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageWestern Blot. Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Rat spleen tissue. All lanes: HIST1H2BC antibody at 1.3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 14 kDa. Observed band size: 14 kDa.
Product ImageChromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2BC (PACO60478) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta -Globin promoter.
Background:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Synonyms:Histone H2B type 1-C/E/F/G/I (Histone H2B.1 A) (Histone H2B.a) (H2B/a) (Histone H2B.g) (H2B/g) (Histone H2B.h) (H2B/h) (Histone H2B.k) (H2B/k) (Histone H2B.l) (H2B/l), HIST1H2BC; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BI, H2BFL; H2BFH; H2BFG; H2BFA; H2BFK
UniProt Protein Function:H2B1C: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 6p22.1

Cellular Component: cytoplasm; extracellular space; nucleoplasm; nucleus

Molecular Function:DNA binding; protein binding

Biological Process: antibacterial humoral response; defense response to Gram-positive bacterium; innate immune response in mucosa; nucleosome assembly

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein has antibacterial and antifungal antimicrobial activity. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
UniProt Code:P62807
NCBI GenInfo Identifier:290457686
NCBI Gene ID:8339
NCBI Accession:P62807.4
UniProt Secondary Accession:P62807,P02278, Q3B872, Q4VB69, Q93078, Q93080,
UniProt Related Accession:P62807
Molecular Weight:13,906 Da
NCBI Full Name:Histone H2B type 1-C/E/F/G/I
NCBI Synonym Full Names:histone cluster 1, H2bg
NCBI Official Symbol:HIST1H2BG  
NCBI Official Synonym Symbols:H2B/a; H2BFA; H2B.1A; dJ221C16.8  
NCBI Protein Information:histone H2B type 1-C/E/F/G/I
UniProt Protein Name:Histone H2B type 1-C/E/F/G/I
UniProt Synonym Protein Names:Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l
Protein Family:Histone
UniProt Gene Name:HIST1H2BC  
UniProt Entry Name:H2B1C_HUMAN
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Anti-Ubiquityl-Histone H2B-K120 Antibody (CAB17949)
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