HIST1H2BC (Ab-116) Antibody (PACO59663)
- SKU:
- PACO59663
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IP
- ChIP
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
HIST1H2BC (Ab-116) Antibody (PACO59663)
The Hist1h2bc Antibody (PACO59663) is a high-quality polyclonal antibody designed for research involving Hist1h2bc, a histone protein involved in gene regulation and chromatin structure. This antibody is raised in rabbits and is highly reactive with human samples, making it ideal for use in various research applications.Hist1h2bc is a member of the histone H2B family, which plays a crucial role in packaging DNA into chromatin and regulating gene expression. This antibody specifically binds to the Hist1h2bc protein, enabling researchers to detect and analyze its expression in different cell types and tissues.
It is validated for use in Western blot applications, allowing for accurate and reliable results.Research involving Hist1h2bc is important in understanding epigenetic modifications and their impact on gene regulation, development, and disease. By studying the function and regulation of Hist1h2bc, researchers can gain valuable insights into processes like transcriptional control, DNA repair, and chromatin remodeling. This antibody is a valuable tool for investigators studying epigenetics, cancer biology, and developmental biology.
| Antibody Name: | HIST1H2BC (Ab-116) Antibody (PACO59663) |
| Antibody SKU: | PACO59663 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IP, ChIP |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:100-1:1000, IP:1:200-1:2000 |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Peptide sequence around site of Lys (116) derived from Human Histone H2B type 1-C/E/F/G/I |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | Antigen Affinity Purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, HL60 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Mouse liver tissue. All lanes: HIST1H2BC antibody at 0.92µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 14 kDa. Observed band size: 14 kDa. |
 | Immunoprecipitating HIST1H2BC in MCF-7 whole cell lysate. Lane 1: Rabbit control IgG instead of PACO59663 in MCF-7 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: PACO59663 (5µg) + MCF-7 whole cell lysate (500µg). Lane 3: MCF-7 whole cell lysate (20µg). |
 | Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H2BC (PACO59663) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta -Globin promoter. |
| Background: | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Synonyms: | Histone H2B type 1-C/E/F/G/I (Histone H2B.1 A) (Histone H2B.a) (H2B/a) (Histone H2B.g) (H2B/g) (Histone H2B.h) (H2B/h) (Histone H2B.k) (H2B/k) (Histone H2B.l) (H2B/l), HIST1H2BC; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BI, H2BFL; H2BFH; H2BFG; H2BFA; H2BFK |
| UniProt Protein Function: | H2B1C: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p22.1 Cellular Component: cytoplasm; extracellular space; nucleoplasm; nucleus Molecular Function:DNA binding; protein binding Biological Process: antibacterial humoral response; defense response to Gram-positive bacterium; innate immune response in mucosa; nucleosome assembly |
| NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein has antibacterial and antifungal antimicrobial activity. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| UniProt Code: | P62807 |
| NCBI GenInfo Identifier: | 290457686 |
| NCBI Gene ID: | 8339 |
| NCBI Accession: | P62807.4 |
| UniProt Secondary Accession: | P62807,P02278, Q3B872, Q4VB69, Q93078, Q93080, |
| UniProt Related Accession: | P62807 |
| Molecular Weight: | 13,906 Da |
| NCBI Full Name: | Histone H2B type 1-C/E/F/G/I |
| NCBI Synonym Full Names: | histone cluster 1, H2bg |
| NCBI Official Symbol: | HIST1H2BGÂ Â |
| NCBI Official Synonym Symbols: | H2B/a; H2BFA; H2B.1A; dJ221C16.8Â Â |
| NCBI Protein Information: | histone H2B type 1-C/E/F/G/I |
| UniProt Protein Name: | Histone H2B type 1-C/E/F/G/I |
| UniProt Synonym Protein Names: | Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l |
| Protein Family: | Histone |
| UniProt Gene Name: | HIST1H2BCÂ Â |
| UniProt Entry Name: | H2B1C_HUMAN |
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