The Acetyl-Histone H2A (K5) Polyclonal Antibody (CAB15620) is a valuable tool for researchers studying histone modifications and their impact on gene regulation. This antibody, raised in rabbits, is highly specific and sensitive for detecting acetylation of histone H2A at lysine 5 in human samples. It has been validated for use in Western blot applications and is suitable for investigating the role of this specific histone modification in various cellular processes.Histone acetylation is a key epigenetic modification that plays a crucial role in regulating gene expression and chromatin structure. Acetylation of specific histone residues, such as lysine 5 on histone H2A, is known to be involved in transcriptional activation and other important cellular functions.
By using the Acetyl-Histone H2A (K5) Polyclonal Antibody, researchers can study the impact of this modification on gene regulation in different cell types and under various experimental conditions.This antibody is an essential tool for researchers interested in epigenetics, chromatin biology, and gene expression regulation. By targeting the acetylation of histone H2A at lysine 5, this antibody provides valuable insights into the mechanisms underlying gene transcription and may have important implications for understanding diseases such as cancer, neurodegenerative disorders, and developmental abnormalities. Researchers can use this antibody to investigate the role of histone acetylation in specific biological processes and
Product Name:
Acetyl-Histone H2A-K5 Rabbit Polyclonal Antibody
SKU:
CAB15620
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic acetylated peptide around K5 of human Histone H2A (NP_003508.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
3012/8329
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Acetyl-Histone H2A-K5 Rabbit pAb (CAB15620) at 1:1000 dilution.C2C12 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
