The His (Histamine) ELISA Kit is specifically designed for the precise measurement of histamine levels in biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it a valuable tool for research in various fields.Histamine is a biogenic amine that plays a crucial role in immune response, inflammatory processes, and neurotransmission. Dysregulation of histamine levels has been associated with allergies, asthma, and other inflammatory disorders, making it a key biomarker for studying these conditions and developing new treatment strategies.
With the His (Histamine) ELISA Kit, researchers can confidently measure histamine levels in biological samples, leading to a better understanding of histamine's role in health and disease. Upgrade your research with this reliable and efficient assay tool.
Product Name:
HIS (Histamine) ELISA Kit (UNES00022)
SKU:
UNES00022
Target:
HIS (Histamine) (UNES00022)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with HIS. During the reaction, HIS in the sample or standard competes with a fixed amount of HIS on the solid phase supporter for sites on the Biotinylated Detection Ab specific to HIS. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HIS in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
88-103
88-100
Average (%)
95
95
94
1:4
Range (%)
85-98
86-101
87-99
Average (%)
91
92
94
1:8
Range (%)
87-98
89-100
87-99
Average (%)
93
95
92
1:16
Range (%)
86-99
90-104
89-102
Average (%)
92
97
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-100
95
EDTA plasma (n=5)
87-100
93
Cell culture media (n=5)
89-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.17
13.32
45.1
5.3
13.54
42.52
Standard deviation
0.27
0.72
1.51
0.28
0.61
1.68
C V (%)
5.22
5.41
3.35
5.28
4.51
3.95
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of HIS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes HIS in samples. No significant cross-reactivity or interference between HIS and analogues was observed.
