The HIRA Polyclonal Antibody (CAB12527) is a valuable tool for researchers studying HIRA, a protein involved in chromatin remodeling and gene regulation. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. It recognizes the HIRA protein, allowing for precise detection and analysis in a variety of cell types.HIRA is known to play a crucial role in DNA replication, repair, and transcription, making it a key player in cellular processes such as development, aging, and cancer.
By targeting HIRA, researchers can gain insights into how chromatin dynamics influence gene expression and cellular functions. This antibody is therefore essential for studies in cell biology, cancer biology, and developmental biology.With its high specificity and reliability, the HIRA Polyclonal Antibody (CAB12527) is an invaluable tool for researchers seeking to unravel the complexities of HIRA-mediated processes and their implications in various biological pathways.
Product Name:
HIRA Rabbit Polyclonal Antibody
SKU:
CAB12527
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 250-550 of human HIRA (NP_003316.3).
This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development.
Purification Method:
Affinity purification
Gene ID:
7290
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HIRA Antibody (CAB12527) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
