The Hikeshi Antibody (PAC036238) is a polyclonal antibody specifically designed for research involving the Hikeshi protein. Hikeshi is a key player in cellular stress response and is known to be involved in regulating heat shock protein levels. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the Hikeshi protein, this antibody enables the detection and analysis of Hikeshi in various cell types, making it an ideal tool for studies in molecular biology and cellular stress research.Hikeshi, also known as C11orf73, is a crucial regulator of cellular responses to stress, such as heat shock and oxidative stress.
Its role in coordinating the cellular stress response pathway makes it a promising target for research in understanding mechanisms of cellular survival and adaptation to stress conditions. The ability to study and manipulate Hikeshi activity can provide insights into potential therapeutic strategies for conditions related to cellular stress and damage. By utilizing the Hikeshi Antibody (PAC036238), researchers can advance their studies on the role of Hikeshi in cellular stress response pathways and its implications in various diseases.
Antibody Name:
HIKESHI Antibody (PACO36238)
Antibody SKU:
PACO36238
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:20-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Protein Hikeshi protein (1-197AA)
Immunohistochemistry of paraffin-embedded human liver cancer using PACO36238 at dilution of 1:100.
Immunohistochemistry of paraffin-embedded human skin tissue using PACO36238 at dilution of 1:100.
Background:
Acts as a specific nuclear import carrier for HSP70 proteins following heat-shock stress: acts by mediating the nucleoporin-dependent translocation of ATP-bound HSP70 proteins into the nucleus. HSP70 proteins import is required to protect cells from heat shock damages. Does not translocate ADP-bound HSP70 proteins into the nucleus.
Synonyms:
Protein Hikeshi, HIKESHI, C11orf73
UniProt Protein Function:
C11orf73: Acts as a specific nuclear import carrier for HSP70 proteins following heat-shock stress: acts by mediating the nucleoporin-dependent translocation of ATP-bound HSP70 proteins into the nucleus. HSP70 proteins import is required to protect cells from heat shock damages. Does not translocate ADP-bound HSP70 proteins into the nucleus. Belongs to the OPI10 family. Following heat-shock treatment. Interacts with ATP-bound HSP70 proteins. Interacts with NUP62 and NUP153 (via F-X-F-G repeats)Protein type: Cell development/differentiationChromosomal Location of Human Ortholog: 11q14.2Cellular Component: cell junction; cytosol; intracellular; nucleoplasm; nucleusMolecular Function: Hsp70 protein binding; protein transporter activityBiological Process: protein import into nucleus; protein transport
UniProt Protein Details:
NCBI Summary:
This gene encodes an evolutionarily conserved nuclear transport receptor that mediates heat-shock-induced nuclear import of 70 kDa heat-shock proteins (Hsp70s) through interactions with FG-nucleoporins. The protein mediates transport of the ATP form but not the ADP form of Hsp70 proteins under conditions of heat shock stress. Structural analyses demonstrate that the protein forms an asymmetric homodimer and that the N-terminal domain consists of a jelly-roll/beta-sandwich fold structure that contains hydrophobic pockets involved in FG-nucleoporin recognition. Reduction of RNA expression levels in HeLa cells using small interfering RNAs results in inhibition of heat shock-induced nuclear accumulation of Hsp70s, indicating a role for this gene in regulation of Hsp70 nuclear import during heat shock stress. [provided by RefSeq, Apr 2016]
