The HIF3A Polyclonal Antibody (CAB20020) is a versatile tool for researchers studying the HIF3A protein, a member of the hypoxia-inducible factor (HIF) family involved in oxygen sensing and response. This antibody, produced in rabbits, exhibits high specificity and sensitivity for human samples, making it suitable for Western blot analysis and other immunological techniques.HIF3A is a key regulator of cellular responses to hypoxia, playing a role in various physiological and pathological processes such as angiogenesis, metabolism, and cancer progression. By targeting HIF3A, researchers can gain insights into these processes and potentially develop new strategies for treating hypoxia-related diseases.
With its reliable performance and broad applicability in different cell types and experimental conditions, the HIF3A Polyclonal Antibody is a valuable asset for studies in molecular biology, oncology, and other fields where hypoxia and HIF signaling are of interest. By enabling the detection and analysis of HIF3A protein levels, this antibody facilitates the elucidation of complex cellular pathways and mechanisms, advancing our understanding of hypoxia biology.
Product Name:
HIF3A Rabbit Polyclonal Antibody
SKU:
CAB20020
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human HIF3A (NP_690008.2).
The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
64344
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of 293F-HIF3A-FlagN, using HIF3A Rabbit pAb (CAB20020) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
