The HIF3A Polyclonal Antibody (PACO05029) is a valuable tool for researchers studying the hypoxia-inducible factor 3 alpha (HIF3A) protein. This antibody, generated in rabbits, is highly reactive with human samples and optimized for Western blot applications. By specifically binding to the HIF3A protein, this antibody enables accurate detection and analysis in a variety of cell types.HIF3A is a member of the hypoxia-inducible factor family and plays a crucial role in cellular responses to low oxygen levels. It is involved in regulating genes responsible for oxygen homeostasis, metabolism, and cell survival.
Research on HIF3A is important for understanding its role in various physiological and pathological conditions, including cancer, cardiovascular diseases, and metabolic disorders.With its high specificity and sensitivity, the HIF3A Polyclonal Antibody (PACO05029) is a valuable tool for researchers investigating the functions and regulation of HIF3A in different biological contexts. By studying HIF3A, researchers can gain insights into the molecular mechanisms underlying oxygen sensing and adaptation, potentially leading to the development of novel therapeutic strategies for hypoxia-related diseases.
Antibody Name:
HIF3A Antibody (PACO05029)
Antibody SKU:
PACO05029
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:20000, WB:1:500-1:2000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthesized peptide derived from the Internal region of human HIF-3 α .
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Synonyms:
Hypoxia-inducible factor 3-α; HIF-3-α; HIF3-α; Basic-helix-loop-helix-PAS protein MOP7; Class E basic helix-loop-helix protein 17; bHLHe17; HIF3-α-1; Inhibitory PAS domain protein; IPAS; Member of PAS protein 7; PAS domain-c
UniProt Protein Function:
HIF3A: Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea. 7 isoforms of the human protein are produced by alternative splicing.Protein type: Transcription factor; Tumor suppressorChromosomal Location of Human Ortholog: 19q13.32Cellular Component: nucleoplasm; cytosolMolecular Function: protein dimerization activity; signal transducer activity; DNA binding; transcription coactivator activity; transcription factor activity; transcription corepressor activityBiological Process: transcription from RNA polymerase II promoter; regulation of transcription from RNA polymerase II promoter; signal transduction
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]
Basic-helix-loop-helix-PAS protein MOP7; Class E basic helix-loop-helix protein 17; bHLHe17; HIF3-alpha-1; Inhibitory PAS domain protein; IPAS; Member of PAS protein 7; PAS domain-containing protein 7
