The HIF1beta/ARNT Rabbit Monoclonal Antibody (CAB19532) is a valuable tool for researchers studying the HIF1beta/ARNT pathway, which plays a critical role in cellular response to hypoxia and is implicated in various diseases, including cancer and ischemic diseases. This antibody, raised in rabbits, has high specificity and sensitivity for detecting HIF1beta and ARNT proteins in human samples, making it ideal for use in Western blot applications.HIF1beta, also known as the beta subunit of Hypoxia-Inducible Factor 1, forms a heterodimer with ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator) to regulate the transcription of genes involved in oxygen homeostasis and response to low oxygen levels.
Dysregulation of the HIF1beta/ARNT pathway has been linked to pathologies such as tumor progression, angiogenesis, and metabolic disorders.By utilizing the HIF1beta/ARNT Rabbit Monoclonal Antibody, researchers can study the protein levels and activity of HIF1beta and ARNT in various cell types and tissues, gaining insights into the mechanisms underlying hypoxia response and potential therapeutic targets for diseases associated with oxygen deprivation. This antibody is a valuable tool for studies in cancer biology, ischemic diseases, and metabolism research.
Product Name:
HIF1β/ARNT Rabbit Monoclonal Antibody
SKU:
CAB19532
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 499-789 of human HIF-1 beta (P27540).
This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
405
Clone Number:
ARC0010
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using HIF1β/ARNT Rabbit mAb (CAB19532) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
