The HIF1AN Polyclonal Antibody (CAB5466) is a valuable tool for research involving the HIF1AN protein, which plays a crucial role in regulating cellular response to hypoxia. This antibody, produced in rabbits, is specifically designed for use in detecting and analyzing HIF1AN in human samples. Validated for Western blot applications, it provides researchers with a reliable means of studying the expression and function of HIF1AN in different cell types.HIF1AN, also known as FIH-1, acts as a negative regulator of the hypoxia inducible factor (HIF) pathway, influencing various cellular processes such as metabolism, angiogenesis, and apoptosis.
Dysregulation of HIF1AN has been implicated in diseases like cancer, cardiovascular disorders, and neurodegenerative conditions, making it a promising target for therapeutic intervention. By gaining insights into the role of HIF1AN in these pathologies, researchers can uncover potential avenues for drug development and treatment strategies.
Enables several functions, including 2-oxoglutarate-dependent dioxygenase activity; NF-kappaB binding activity; and transition metal ion binding activity. Involved in several processes, including negative regulation of Notch signaling pathway; negative regulation of transcription from RNA polymerase II promoter in response to hypoxia; and protein hydroxylation. Located in cytosol; nucleoplasm; and perinuclear region of cytoplasm. Colocalizes with nucleus.
Purification Method:
Affinity purification
Gene ID:
55662
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] HIF1AN/FIH1 Rabbit pAb (CAB5466) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
