The HFE Polyclonal Antibody (CAB1310) is specifically designed for research involving the HFE protein, a key regulator of iron homeostasis in the body. The antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the HFE protein, this antibody enables accurate detection and analysis in various cell types, making it an essential tool for studies in the fields of hematology, iron metabolism, and related disorders.HFE, also known as the hemochromatosis protein, is crucial for the regulation of iron absorption and distribution in the body.
Mutations in the HFE gene can lead to iron overload and contribute to conditions such as hereditary hemochromatosis. Research on HFE is vital for understanding iron metabolism and developing treatments for iron-related disorders.Overall, the HFE Polyclonal Antibody (CAB1310) is a valuable tool for researchers studying iron homeostasis, hemochromatosis, and related conditions. Its high reactivity and specificity make it an essential component in the study of HFE protein function and its implications in human health and disease.
Product Name:
HFE Rabbit Polyclonal Antibody
SKU:
CAB1310
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 80-306 of human HFE (NP_000401.1).
Cell membrane, Single-pass type I membrane protein.
Calculated MW:
40kDa
Observed MW:
50kDa
The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene.
Purification Method:
Affinity purification
Gene ID:
3077
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HFE antibody (CAB1310) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
