The Hexokinase II Monoclonal Antibody is a vital tool for researchers studying the hexokinase II protein, a key enzyme involved in glucose metabolism and cellular energy production. This antibody, produced using a monoclonal antibody technology, exhibits high specificity and sensitivity towards human samples, making it an excellent choice for various experimental techniques including Western blotting and immunohistochemistry.Hexokinase II is a crucial player in the regulation of glucose metabolism and its dysregulation has been linked to various diseases including cancer, diabetes, and neurodegenerative disorders.
By targeting the hexokinase II protein, researchers can gain insights into its role in disease progression and potentially develop new therapeutic strategies.This monoclonal antibody provides researchers with a reliable tool to detect and analyze hexokinase II expression in different cell types and tissues, aiding in the understanding of its function and significance in various physiological and pathological conditions. Its versatility and specificity make it an invaluable resource for studies in metabolic disorders, cancer biology, and drug development.
Product Name:
Hexokinase II Monoclonal Antibody
SKU:
CAB22319
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Hexokinase II (NP_000180.2).
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
Purification Method:
Affinity purification
Gene ID:
3099
Clone Number:
ARC52057
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts from normal (control) and HexokinaseII knockout (KO) HeLa(KO) cells, using HexokinaseII antibody (CAB22319) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
