The Hexokinase 2 Polyclonal Antibody (CAB0994) is a valuable tool for researchers studying hexokinase 2, an enzyme involved in glucose metabolism and energy production in cells. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. It specifically binds to the hexokinase 2 protein, allowing for the detection and analysis of this important enzyme in a variety of cell types.Hexokinase 2 is known to play a key role in the regulation of glucose metabolism, particularly in cancer cells which rely heavily on glycolysis for energy production.
By targeting hexokinase 2 with this antibody, researchers can gain valuable insights into the role of this enzyme in cancer metabolism and potentially identify new therapeutic targets for cancer treatment.Overall, the Hexokinase 2 Polyclonal Antibody (CAB0994) is an essential tool for studies in cancer biology, metabolism, and energy regulation, providing researchers with the ability to investigate the function of hexokinase 2 in various cellular processes and disease states.
Product Name:
[KO Validated] HK2 Rabbit Polyclonal Antibody
SKU:
CAB0994
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Hexokinase II (NP_000180.2).
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
Purification Method:
Affinity purification
Gene ID:
3099
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Hexokinase II Rabbit pAb (CAB0994) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
