The Hexokinase-1 Polyclonal Antibody (CAB1054) is a valuable tool for researchers studying Hexokinase-1, an enzyme involved in glucose metabolism and energy production within cells. This antibody, developed using rabbit immunization, exhibits high reactivity with human samples and has been specifically validated for use in Western blot applications.Hexokinase-1 plays a crucial role in catalyzing the first step of glucose metabolism, converting glucose to glucose-6-phosphate. Dysregulation of Hexokinase-1 activity has been linked to various diseases, including cancer and diabetes, making it an attractive target for therapeutic development and research.
By targeting the Hexokinase-1 protein, researchers can gain insights into its expression levels and function in different cell types, providing valuable information for studies in metabolism, cancer biology, and other related fields. This antibody enables accurate detection and analysis of Hexokinase-1, making it an essential tool for advancing our understanding of cellular energy metabolism and its implications in health and disease.
Product Name:
[KO Validated] HK1 Rabbit Polyclonal Antibody
SKU:
CAB1054
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 21-219 of human HK1 (NP_000179.2).
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.
Purification Method:
Affinity purification
Gene ID:
3098
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] HK1 Rabbit pAb (CAB1054) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
