The Hes7 Antibody (PAC059513) is a valuable tool for researchers studying the Hes7 protein, a key player in embryonic development and cellular differentiation. This polyclonal antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in immunohistochemistry and Western blot applications.Hes7 is a critical regulator of the Notch signaling pathway, controlling various cellular processes such as cell fate determination and tissue patterning. Dysregulation of Hes7 expression has been implicated in developmental disorders and certain types of cancer. By targeting the Hes7 protein, researchers can gain insights into its function and potentially uncover new therapeutic targets for diseases related to abnormal cell growth and differentiation.
This Hes7 antibody enables researchers to detect and analyze the Hes7 protein in diverse cell types, providing valuable information for developmental biology and cancer research. Its specificity and sensitivity make it a reliable tool for studying the molecular mechanisms underlying embryogenesis and tumorigenesis. Gain a deeper understanding of Hes7 and its involvement in crucial biological processes with this high-quality antibody.
Antibody Name:
HES7 Antibody (PACO59513)
Antibody SKU:
PACO59513
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:200-1:500
Species Reactivity:
Human
Immunogen:
Recombinant Human Transcription factor HES-7 protein (127-192AA)
IHC image of PACO59513 diluted at 1:400 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Transcriptional repressor. Represses transcription from both N box- and E box-containing promoters. May with HES1, cooperatively regulate somite formation in the presomitic mesoderm (PSM). May function as a segmentation clock, which is essential for coordinated somite segmentation (By similarity).
Synonyms:
Transcription factor HES-7 (hHes7) (Class B basic helix-loop-helix protein 37) (bHLHb37) (Hairy and enhancer of split 7) (bHLH factor Hes7), HES7, BHLHB37
UniProt Protein Function:
HES7: Transcriptional repressor. Represses transcription from both N box- and E box-containing promoters. May with HES1, cooperatively regulate somite formation in the presomitic mesoderm (PSM). May function as a segmentation clock, which is essential for coordinated somite segmentation. Defects in HES7 are the cause of spondylocostal dysostosis type 4 (SCDO4). A rare condition of variable severity characterized by vertebral and costal anomalies. The main feature include dwarfism, vertebral fusion, hemivertebrae, posterior rib fusion, reduced rib number, and other rib malformations.Chromosomal Location of Human Ortholog: 17p13.1Disease: Spondylocostal Dysostosis 4, Autosomal Recessive
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the hairy and enhancer of split family of bHLH transcription factors. The mouse ortholog of this gene is regulated by Notch signaling. The protein functions as a transcriptional repressor, and is implicated in correct patterning of the axial skeleton. A mutation in this gene has been shown to result in spondylocostal dysostosis. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
