The HES1 Rabbit Monoclonal Antibody is a highly specific reagent designed for the study of HES1, a transcription factor involved in various developmental processes and cell fate determination. This antibody, produced through rabbit monoclonal technology, is well-suited for use in various applications, including immunofluorescence, immunohistochemistry, and flow cytometry.HES1 is known to play a key role in regulating stem cell maintenance and differentiation, making it a crucial target for research in developmental biology and cancer biology. The HES1 Rabbit Monoclonal Antibody allows for precise detection and visualization of HES1 protein expression in different cell types and tissues, providing valuable insights into its function and mechanisms of action.
By using the HES1 Rabbit Monoclonal Antibody, researchers can further investigate the role of HES1 in embryogenesis, tissue regeneration, and disease pathogenesis. Its high specificity and sensitivity make it an essential tool for studies aiming to understand the intricate regulatory networks controlled by HES1 and its implications in various physiological and pathological processes.
Product Name:
HES1 Rabbit Monoclonal Antibody
SKU:
CAB0925
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 181-280 of human HES1 (Q14469).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Synonyms:
HHL; HRY; HES-1; bHLHb39; HES1
Positive Sample:
Mouse eye,Rat testis
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
30kDa
Observed MW:
30kDa
This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
Purification Method:
Affinity purification
Gene ID:
3280
Clone Number:
ARC0513
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using HES1 Rabbit mAb (CAB0925) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
