HER2/ErbB2 Polyclonal Antibody (CAB21768)
- SKU:
- CAB21768
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
Description
| Product Name: | HER2/ErbB2 Polyclonal Antibody |
| SKU: | CAB21768 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1156-1255 of human HER2/ErbB2 (NP_004439.2). |
| Sequence: | PLPA ARPA GATL ERPK TLSP GKNG VVKD VFAF GGAV ENPE YLTP QGGA APQP HPPP AFSP AFDN LYYW DQDP PERG APPS TFKG TPTA ENPE YLGL DVPV |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:100 - 1:500 |
| Synonyms: | NEU; NGL; HER2; TKR1; CD340; HER-2; VSCN2; MLN 19; MLN-19; c-ERB2; c-ERB-2; HER-2/neu; p185(erbB2); B2 |
| Positive Sample: | HeLa,MCF7 |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cell membrane, Cytoplasm, Nucleus, Nucleus, Single-pass type I membrane protein, perinuclear region. |
| Calculated MW: | 138kDa |
| Observed MW: | 185kDa |
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.
| Purification Method: | Affinity purification |
| Gene ID: | 2064 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from MCF7 cells, using [KO Validated] HER2/ErbB2 Rabbit pAb (CAB21768) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s. |
