The Heme Oxygenase-1 Polyclonal Antibody (CAB1346) is a valuable tool for researchers studying heme oxygenase-1, an enzyme involved in the degradation of heme and regulation of oxidative stress. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to heme oxygenase-1, researchers can detect and analyze this enzyme in a variety of cell types, making it an ideal choice for studies in oxidative stress, inflammation, and diseases such as cardiovascular disease and cancer.
Heme oxygenase-1 (HO-1) is a key regulator of cellular oxidative stress and is known to have cytoprotective and anti-inflammatory properties. Dysregulation of HO-1 has been implicated in a number of pathological conditions, including diabetes, neurodegenerative diseases, and ischemic injury. By studying the expression and activity of HO-1 using this antibody, researchers can gain valuable insights into the role of this enzyme in various disease processes and potentially identify new avenues for therapeutic intervention.
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.
Purification Method:
Affinity purification
Gene ID:
3162
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from A549 cells, using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (CAB1346) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
