The Heme Oxygenase-1 Polyclonal Antibody (CAB11102) is an essential tool for researchers studying heme oxygenase-1 (HO-1), an enzyme involved in maintaining cellular homeostasis and protecting against oxidative stress. Raised in rabbits, this antibody is highly specific for human samples and has been validated for use in Western blot applications. By binding to the HO-1 protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it a valuable asset for investigations in oxidative stress, inflammation, and diseases like cancer and cardiovascular disorders.HO-1, a stress-responsive enzyme, plays a crucial role in the breakdown of heme into biliverdin, carbon monoxide, and iron, leading to the antioxidant and cytoprotective effects that are essential for cellular survival under oxidative conditions.
Dysfunction of HO-1 has been linked to various pathologies, highlighting its potential as a therapeutic target in conditions involving oxidative damage and inflammatory responses. By studying the expression and regulation of HO-1, researchers can gain insights into mechanisms of cellular protection and develop novel strategies for combating oxidative stress-related diseases.
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.
Purification Method:
Affinity purification
Gene ID:
3162
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse spleen, using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (CAB11102) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
