Anti-HECW2 Antibody (PACO59525)

Antibody Name:	HECW2 Antibody (PACO59525)
Antibody SKU:	PACO59525
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human E3 ubiquitin-protein ligase HECW2 protein (495-641AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, 293T whole cell lysate. All lanes: HECW2 antibody at 3.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 176, 136 kDa. Observed band size: 176 kDa.
	IHC image of PACO59525 diluted at 1:500 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
	IHC image of PACO59525 diluted at 1:500 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:	E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73. Involved in the regulation of mitotic metaphase/anaphase transition.
Synonyms:	E3 ubiquitin-protein ligase HECW2 (EC 2.3.2.26) (HECT, C2 and WW domain-containing protein 2) (HECT-type E3 ubiquitin transferase HECW2) (NEDD4-like E3 ubiquitin-protein ligase 2), HECW2, KIAA1301 NEDL2

UniProt Protein Function:	HECW2: E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Ubiquitin ligase; EC 6.3.2.19; EC 6.3.2.-; Ligase; Ubiquitin conjugating systemChromosomal Location of Human Ortholog: 2q32.3Cellular Component: cytoplasm; nucleusMolecular Function: ubiquitin-protein ligase activityBiological Process: protein ubiquitination during ubiquitin-dependent protein catabolic process
UniProt Protein Details:
NCBI Summary:
UniProt Code:	Q9P2P5
NCBI GenInfo Identifier:	126215718
NCBI Gene ID:	57520
NCBI Accession:	Q9P2P5.2
UniProt Secondary Accession:	Q9P2P5,Q17RT5, Q68DF8, Q9NPS9, B8ZZB4
UniProt Related Accession:	Q9P2P5
Molecular Weight:	135,905 Da
NCBI Full Name:	E3 ubiquitin-protein ligase HECW2
NCBI Synonym Full Names:	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
NCBI Official Symbol:	HECW2
NCBI Official Synonym Symbols:	NEDL2
NCBI Protein Information:	E3 ubiquitin-protein ligase HECW2
UniProt Protein Name:	E3 ubiquitin-protein ligase HECW2
UniProt Synonym Protein Names:	HECT, C2 and WW domain-containing protein 2; NEDD4-like E3 ubiquitin-protein ligase 2
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	HECW2
UniProt Entry Name:	HECW2_HUMAN

Antibodies

Secondary Antibody

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