The HECW1 Polyclonal Antibody (PAC063991) is a powerful tool for researchers studying the HECW1 protein, which plays a key role in the regulation of cellular processes, including protein degradation and DNA repair. This high-quality antibody, raised in rabbits, has been validated for use in Western blot applications and is highly reactive with human samples.HECW1, a HECT domain-containing E3 ubiquitin ligase, is involved in the ubiquitination of target proteins, leading to their degradation or modification. Its function in protein turnover makes it essential for maintaining cellular homeostasis and regulating various signaling pathways. This antibody specifically binds to the HECW1 protein, allowing for accurate detection and analysis in a variety of cell types.
Research on HECW1 is important for understanding its role in processes such as cell growth, differentiation, and DNA damage response. Dysregulation of HECW1 has been implicated in various diseases, including cancer and neurodegenerative disorders. By investigating the function of HECW1, researchers can uncover potential targets for therapeutic interventions and gain insights into the molecular mechanisms underlying these conditions. Use the HECW1 Polyclonal Antibody to advance your studies in molecular biology, cancer research, and beyond.
Antibody Name:
HECW1 Antibody (PACO63991)
Antibody SKU:
PACO63991
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IF
Recommended Dilutions:
ELISA:1:2000-1:10000, IF:1:50-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human E3 ubiquitin-protein ligase HECW1 protein (751-900AA)
Immunofluorescence staining of A549 cells with PACO63991 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent degradation of DVL1. Also targets the mutant SOD1 protein involved in familial amyotrophic lateral sclerosis (FALS). Forms cytotoxic aggregates with DVL1, SSR3 and mutant SOD1 that lead to motor neuron death in FALS.
