The HDAC8 Polyclonal Antibody (CAB5829) is a valuable tool for research involving HDAC8, a key enzyme in the regulation of gene expression and cell cycle progression. This antibody, produced in rabbits, is highly specific for human samples and is validated for use in Western blot applications. By targeting the HDAC8 protein, it allows for precise detection and analysis in a variety of cell types, making it ideal for studies in epigenetics, cancer biology, and drug development.
HDAC8, a member of the histone deacetylase family, plays a critical role in the modification of chromatin structure and the regulation of gene transcription. Dysregulation of HDAC8 has been implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. Research into the function and regulation of HDAC8 is essential for understanding its role in disease pathogenesis and identifying potential therapeutic targets for intervention.
Product Name:
[KO Validated] HDAC8 Rabbit Polyclonal Antibody
SKU:
CAB5829
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human HDAC8 (NP_060956.1).
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
55869
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and HDAC8 knockout (KO) HeLa cells, using [KO Validated] HDAC8 Rabbit pAb (CAB5829) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
