The HDAC5 Polyclonal Antibody (CAB0632) is a valuable tool for researchers studying histone deacetylase 5 (HDAC5), a protein involved in epigenetic regulation and gene expression. This antibody, generated in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blotting applications. By specifically binding to HDAC5, researchers can accurately detect and analyze the protein in various cell types, making it a versatile option for studies in epigenetics and gene regulation.HDAC5 is a key player in the epigenetic control of gene expression, impacting various cellular processes such as cell cycle progression, differentiation, and apoptosis.
Dysregulation of HDAC5 has been linked to several diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. By investigating the role of HDAC5 in these conditions, researchers can gain insights into potential therapeutic strategies targeting epigenetic modifications.Overall, the HDAC5 Polyclonal Antibody provides researchers with a reliable tool for exploring the functions and mechanisms of HDAC5 in various biological contexts. Its specificity and sensitivity make it an essential component in studies aiming to unravel the complex interplay between epigenetic regulation and disease development.
Product Name:
HDAC5 Rabbit Polyclonal Antibody
SKU:
CAB0632
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 500-600 of human HDAC5 (NP_005465.2).
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
10014
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HDAC5 antibody (CAB0632) at 1:200 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
