The HDAC5/HDAC9 Antibody (PACO06541) is a versatile tool for researchers studying histone deacetylase proteins HDAC5 and HDAC9. These proteins play key roles in gene expression regulation, cell differentiation, and DNA repair processes. The antibody, generated in rabbits, is highly specific and suitable for use in various applications such as Western blotting and immunofluorescence.HDAC5 and HDAC9 are important targets in cancer research, as their dysregulation has been linked to tumor progression and metastasis. By using this antibody, researchers can effectively analyze the expression levels and localization of HDAC5 and HDAC9 in different cell types and tissues.
This information is crucial for understanding the molecular mechanisms underlying cancer development and progression.In addition, the HDAC5/HDAC9 Antibody is also valuable for studies focusing on neurodegenerative diseases, cardiovascular disorders, and metabolic syndromes. With its high specificity and sensitivity, this antibody enables accurate detection and analysis of HDAC5 and HDAC9, offering insights into their functions and potential therapeutic targets in various pathological conditions.
Antibody Name:
HDAC5/HDAC9 Antibody (PACO06541)
Antibody SKU:
PACO06541
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB, IHC
Recommended Dilutions:
ELISA:1:5000, WB:1:500-1:2000, IHC:1:100-1:300
Species Reactivity:
Human, Mouse
Immunogen:
Synthesized peptide derived from human HDAC5/9 around the non-phosphorylation site of S259/220.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western Blot analysis of NIH-3T3 RAT-intestines cells using HDAC5/9 Polyclonal Antibody.
Western Blot analysis of HepG2 cells using HDAC5/9 Polyclonal Antibody.
HDAC5: a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Coimmunoprecipitates only with HDAC3 family members. Interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. Two alternatively spliced isoforms have been described.
Molecular Function:protein binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein kinase C binding; NAD-dependent histone deacetylase activity (H3-K14 specific); metal ion binding; histone deacetylase binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; transcription factor binding; transcription corepressor activity
Biological Process: response to drug; regulation of skeletal muscle fiber development; establishment and/or maintenance of chromatin architecture; Notch signaling pathway; transcription, DNA-dependent; B cell activation; heart development; chromatin silencing; histone deacetylation; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; response to cocaine; regulation of gene expression, epigenetic; osteoblast development; chromatin remodeling; cellular response to insulin stimulus; protein amino acid deacetylation; B cell differentiation; regulation of protein binding; positive regulation of transcription factor activity; negative regulation of osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; negative regulation of transcription, DNA-dependent; multicellular organismal response to stress
NCBI Summary:
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
