The HDAC2 Rabbit Monoclonal Antibody (CAB19626) is a valuable tool for researchers studying the histone deacetylase 2 (HDAC2) protein, which plays a crucial role in epigenetic regulation and gene expression. This antibody, generated in rabbits, is highly specific and sensitive for use in various applications, including Western blotting, immunofluorescence, and immunohistochemistry.HDAC2 is known to be involved in chromatin remodeling and transcriptional regulation, making it an important target for studies in cancer, neurodegenerative diseases, and other conditions where epigenetic dysregulation occurs.
By detecting and quantifying HDAC2 levels in cells and tissues, researchers can gain valuable insights into its function and potential therapeutic applications.The validation of this antibody for use in multiple experimental techniques makes it a versatile tool for investigating HDAC2 function in different biological contexts. Its high affinity and specificity ensure reliable and reproducible results, making it a valuable asset for research labs focusing on epigenetics and gene regulation.
Product Name:
HDAC2 Rabbit Monoclonal Antibody
SKU:
CAB19626
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HDAC2 (NP_001518.3).
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
HD2; RPD3; YAF1; KDAC2; HDAC2
Positive Sample:
HeLa,293T,NIH/3T3,C6,Mouse liver
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
55kDa
Observed MW:
60kDa
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
3066
Clone Number:
ARC2647
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using HDAC2 Rabbit mAb (CAB19626) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
