The HDAC2 Polyclonal Antibody (CAB2084) is a valuable tool for researchers studying HDAC2, a key enzyme involved in regulating gene expression through histone deacetylation. This antibody, generated in rabbits, exhibits high specificity and sensitivity for human samples, making it suitable for Western blot applications.HDAC2, belonging to the histone deacetylase family, plays a vital role in epigenetic regulation by modifying chromatin structure and gene transcription. Dysregulation of HDAC2 has been linked to various diseases, including cancer, neurological disorders, and inflammatory conditions.
By targeting HDAC2 with this antibody, researchers can investigate its function and potential as a therapeutic target in these disease settings.The ability to accurately detect and quantify HDAC2 levels in different cell types provides valuable insights into its biological functions and regulatory mechanisms. This antibody is a valuable tool for studies in epigenetics, cancer biology, and drug discovery, facilitating a deeper understanding of HDAC2 in health and disease.
Product Name:
HDAC2 Rabbit Polyclonal Antibody
SKU:
CAB2084
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 390-488 of human HDAC2 (NP_001518.3).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
HD2; RPD3; YAF1; KDAC2; HDAC2
Positive Sample:
K-562,NIH/3T3,COS-1,COS-7,HeLa,Jurkat,SH-SY5Y
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
55kDa
Observed MW:
60kDa
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
3066
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using HDAC2 Rabbit pAb (CAB2084) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
