The HDAC1 Polyclonal Antibody (CAB0238) is a valuable tool for researchers studying histone deacetylase 1 (HDAC1), a critical enzyme involved in regulating gene expression through chromatin remodeling. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.HDAC1 is known to play a vital role in various biological processes, including cell cycle regulation, apoptosis, and differentiation. Dysregulation of HDAC1 has been implicated in several diseases, such as cancer, neurodegenerative disorders, and inflammatory conditions.
By targeting HDAC1 with this antibody, researchers can better understand the mechanisms underlying these diseases and potentially identify new therapeutic targets.The HDAC1 Polyclonal Antibody (CAB0238) provides reliable detection and analysis of HDAC1 protein levels in different cell types, making it an ideal tool for studies in epigenetics, cancer biology, and drug development. By utilizing this antibody, researchers can gain insights into the role of HDAC1 in disease progression and potentially develop novel treatment strategies to target this critical enzyme.
Product Name:
[KO Validated] HDAC1 Rabbit Polyclonal Antibody
SKU:
CAB0238
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 393-482 of human HDAC1 (NP_004955.2).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
HD1; RPD3; KDAC1; GON-10; RPD3L1; C1
Positive Sample:
293T,HeLa,NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
55kDa
Observed MW:
62kDa/
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
Purification Method:
Affinity purification
Gene ID:
3065
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KD Validated] HDAC1 Rabbit pAb (CAB0238) at 1:2000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020)Exposure time: 20 s.
