The HCRTR1 Polyclonal Antibody (CAB14740) is a vital tool for researchers studying the HCRTR1 protein, also known as the hypocretin receptor 1. This cell surface receptor is involved in the regulation of sleep and wakefulness, making it a key target for studies on sleep disorders and related conditions.Raised in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the HCRTR1 protein, researchers can accurately detect and analyze its expression in various cell types, providing valuable insights into the mechanisms underlying sleep regulation.The HCRTR1 antibody is particularly valuable for investigating the role of hypocretin signaling in sleep disorders such as narcolepsy, insomnia, and circadian rhythm disruptions.
By understanding the function of HCRTR1, researchers can develop targeted therapies to improve sleep quality and address the underlying causes of these conditions.Overall, the HCRTR1 Polyclonal Antibody (CAB14740) is a crucial tool for researchers seeking to unravel the mysteries of sleep regulation and develop effective treatments for sleep-related disorders. Its specificity, sensitivity, and reliability make it an essential asset for studies in neuroscience, sleep medicine, and related fields.
Product Name:
HCRTR1 Rabbit Polyclonal Antibody
SKU:
CAB14740
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human HCRTR1 (NP_001516.2).
Sequence:
HPLL FKST ARRA RGSI LGIW AVSL AIMV PQAA VMEC SSVL PELA NRTR LFSV CDER WADD LYPK IYHS CFFI VTYL APLG LMAM AYFQ IFRK LWGR QIPG T
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Synonyms:
OX1R; OXR1; ORXR1; HCRTR1
Positive Sample:
C6
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Multi-pass membrane protein.
Calculated MW:
48kDa
Observed MW:
48kDa
The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-protein coupled receptor that binds orexin A and orexin B.
Purification Method:
Affinity purification
Gene ID:
3061
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using HCRTR1 Rabbit pAb (CAB14740) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
