The HCoV-229E Spike S2 Monoclonal Antibody is a specialized antibody designed for research involving the Human Coronavirus HCoV-229E, specifically targeting the Spike S2 protein. This antibody, produced through monoclonal techniques, is highly specific and reactive with human samples, making it an ideal tool for studying the mechanisms of infection and immune response against this coronavirus strain.The Spike S2 protein of HCoV-229E is a critical component involved in viral entry into host cells and plays a key role in the infectivity and pathogenesis of the virus. By targeting this protein with the monoclonal antibody, researchers can elucidate the molecular interactions between the virus and host cells, paving the way for the development of novel therapeutics and vaccines against HCoV-229E.
This monoclonal antibody is validated for use in various research applications, including immunofluorescence, immunohistochemistry, and ELISA, providing researchers with a versatile tool for studying HCoV-229E biology. Its high specificity and reactivity make it a valuable asset in the fields of virology, immunology, and infectious disease research. By understanding the role of the Spike S2 protein in HCoV-229E infection, researchers can gain insights into potential targets for antiviral interventions and strategies to combat emerging coronavirus strains.
Product Name:
HCoV-229E Spike S2 Monoclonal Antibody
SKU:
CAB22604
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
HCoV-229E
Immunogen:
Recombinant protein of human HCoV-229E Spike S2.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:10000 - 1:80000
Positive Sample:
Human coronavirus (HCoV-229E) Spike Protein (S1+S2 ECD His Tag)
Conjugate:
Unconjugated
Calculated MW:
129kDa
Observed MW:
180kDa
S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
Purification Method:
Affinity purification
Gene ID:
918758
Clone Number:
ARC57280
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of Human coronavirus (HCoV-229E) Spike Protein (S1+S2 ECD His Tag), using HCoV-229E Spike S2 antibody (CAB22604) at 1:70000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
