The HCoV-229E Spike S1 Monoclonal Antibody is a specialized antibody designed for research involving the HCoV-229E Spike protein, a key component of the human coronavirus HCoV-229E. This monoclonal antibody, developed for use in various immunological applications, is highly specific and reactive with human samples.The HCoV-229E Spike S1 Monoclonal Antibody is validated for use in techniques such as Western blotting, immunofluorescence, and immunohistochemistry. By targeting the Spike protein of the HCoV-229E virus, this antibody enables researchers to study the virus's structure, function, and interactions with host cells.
Understanding the role of the HCoV-229E Spike protein is essential for developing effective diagnostics, treatments, and vaccines for diseases caused by this virus. This antibody provides a valuable tool for investigating the biology of HCoV-229E and its implications for public health.In conclusion, the HCoV-229E Spike S1 Monoclonal Antibody is a crucial reagent for research into human coronavirus HCoV-229E and holds promise for advancing our understanding of this important pathogen.
Product Name:
HCoV-229E Spike S1 Monoclonal Antibody
SKU:
CAB22605
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
HCoV-229E
Immunogen:
Recombinant protein of human HCoV-229E Spike S1.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Positive Sample:
Human coronavirus (HCoV-229E) Spike Protein (S1+S2 ECDHis Tag)
Conjugate:
Unconjugated
Calculated MW:
129kDa
Observed MW:
160kDa
S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
Purification Method:
Affinity purification
Gene ID:
918758
Clone Number:
ARC57845
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using HCoV-229E Spike S1 antibody (CAB22605) at 1:800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
