Anti-HCAR2 Antibody (CAB15611)
- SKU:
- CAB15611
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
Anti-HCAR2 Antibody (CAB15611)
The HCAR2 Polyclonal Antibody (CAB15611) is a specialized tool for researchers studying HCAR2, a G protein-coupled receptor known for its role in immune modulation and metabolic regulation. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the HCAR2 protein, this antibody enables precise detection and analysis in a variety of cell types, making it a valuable asset for investigations in immunology, metabolism, and beyond.HCAR2, also known as hydroxycarboxylic acid receptor 2, plays a crucial role in sensing nutrients and signaling metabolic responses in the body.
Its involvement in immune regulation suggests potential implications for conditions such as inflammation, metabolic disorders, and even certain types of cancer. By studying the function of HCAR2, researchers may uncover new insights into these diseases and potentially identify novel therapeutic targets for intervention.Unlock the potential of your research with the HCAR2 Polyclonal Antibody (CAB15611) and delve into the intricate world of immune and metabolic regulation.
| Antibody Name: | Anti-HCAR2 Antibody |
| Antibody SKU: | CAB15611 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 250-350 of human HCAR2 (NP_808219.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, Rat heart |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 250-350 of human HCAR2 (NP_808219.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VRIR IFWL LHTS GTQN CEVY RSVD LAFF ITLS FTYM NSML DPVV YYFS SPSF PNFF STLI NRCL QRKM TGEP DNNR STSV ELTG DPNK TRGA PEAL MANS G |
| Gene ID: | 338442 |
| Uniprot: | Q8TDS4 |
| Cellular Location: | |
| Calculated MW: | 42kDa |
| Observed MW: | 42kDa |
| Synonyms: | HCAR2, GPR109A, HCA2, HM74a, HM74b, NIACR1, PUMAG, Puma-g |
| Background: |
| UniProt Protein Function: | GPR109A: Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5- methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide. Belongs to the G-protein coupled receptor 1 family. |
| UniProt Protein Details: | Protein type:Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: cell junction; plasma membrane Biological Process: negative regulation of lipid catabolic process; neutrophil apoptosis; positive regulation of neutrophil apoptosis |
| UniProt Code: | Q8TDS4 |
| NCBI GenInfo Identifier: | 74762622 |
| NCBI Gene ID: | 338442 |
| NCBI Accession: | Q8TDS4.1 |
| UniProt Secondary Accession: | Q8TDS4,A0PJL5, A7LGG3, |
| UniProt Related Accession: | Q8TDS4 |
| Molecular Weight: | 41,850 Da |
| NCBI Full Name: | Hydroxycarboxylic acid receptor 2 |
| NCBI Synonym Full Names: | hydroxycarboxylic acid receptor 2 |
| NCBI Official Symbol: | HCAR2Â Â |
| NCBI Official Synonym Symbols: | HCA2; HM74a; HM74b; PUMAG; NIACR1; Puma-g; GPR109AÂ Â |
| NCBI Protein Information: | hydroxycarboxylic acid receptor 2 |
| UniProt Protein Name: | Hydroxycarboxylic acid receptor 2 |
| UniProt Synonym Protein Names: | G-protein coupled receptor 109A; G-protein coupled receptor HM74A; Niacin receptor 1; Nicotinic acid receptor |
| Protein Family: | Hydroxycarboxylic acid receptor |
| UniProt Gene Name: | HCAR2Â Â |
| UniProt Entry Name: | HCAR2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HCAR2 antibody (CAB15611) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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