HBG2 Polyclonal Antibody (CAB7683)
- SKU:
- CAB7683
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
Frequently bought together:
Description
| Product Name: | HBG2 Polyclonal Antibody |
| SKU: | CAB7683 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HBG2 (NP_000175.1). |
| Sequence: | MGHF TEED KATI TSLW GKVN VEDA GGET LGRL LVVY PWTQ RFFD SFGN LSSA SAIM GNPK VKAH GKKV LTSL GDAI KHLD DLKG TFAQ LSEL HCDK LHVD |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:100 - 1:500 |
| Synonyms: | TNCY; HBG-T1; HBG2 |
| Positive Sample: | K-562 |
| Conjugate: | Unconjugated |
| Cellular Localization: | blood microparticle, cytosol, hemoglobin complex |
| Calculated MW: | 16kDa |
| Observed MW: | 13kDa |
The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'- epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.
| Purification Method: | Affinity purification |
| Gene ID: | 3048 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
 | Western blot analysis of extracts of K-562 cells, using HBG2 antibody (CAB7683) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s. |
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