The HBG2 Polyclonal Antibody is a versatile tool for researchers studying the HBG2 protein, which is involved in fetal hemoglobin regulation and erythropoiesis. This antibody, generated in rabbits, is highly specific and reactive with human samples, making it reliable for use in various immunoassays such as Western blot and immunohistochemistry.HBG2, also known as fetal hemoglobin subunit gamma, plays a crucial role in the production of fetal hemoglobin during embryonic development. Dysregulation of HBG2 expression has been linked to various hematological disorders, making it a promising target for therapeutic interventions.
By using the HBG2 Polyclonal Antibody, researchers can detect and analyze HBG2 protein levels in different cell types, contributing to a better understanding of its function and potential implications in disease pathology.Overall, the HBG2 Polyclonal Antibody offers a reliable and effective tool for researchers interested in studying fetal hemoglobin regulation, erythropoiesis, and related disorders. Its high specificity and sensitivity make it a valuable asset in the field of hematology research.
Product Name:
HBG2 Polyclonal Antibody
SKU:
CAB7683
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HBG2 (NP_000175.1).
Sequence:
MGHF TEED KATI TSLW GKVN VEDA GGET LGRL LVVY PWTQ RFFD SFGN LSSA SAIM GNPK VKAH GKKV LTSL GDAI KHLD DLKG TFAQ LSEL HCDK LHVD
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Synonyms:
TNCY; HBG-T1; HBG2
Positive Sample:
K-562
Conjugate:
Unconjugated
Cellular Localization:
blood microparticle, cytosol, hemoglobin complex
Calculated MW:
16kDa
Observed MW:
13kDa
The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'- epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.
Purification Method:
Affinity purification
Gene ID:
3048
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of K-562 cells, using HBG2 antibody (CAB7683) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
