The HBG1/HBG2 Polyclonal Antibody is a valuable tool for researchers studying hemoglobin gamma chains HBG1 and HBG2. These hemoglobin chains are important in the development of red blood cells and are typically expressed in the fetal stage of development. The antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blotting applications.By specifically binding to the HBG1 and HBG2 proteins, this antibody allows for the detection and analysis of these important hemoglobin chains in a variety of cell types.
This makes it an ideal choice for studies focusing on hematopoiesis, erythropoiesis, and disorders related to abnormal hemoglobin production.The HBG1/HBG2 Polyclonal Antibody can provide valuable insight into the function and regulation of hemoglobin gamma chains, shedding light on their roles in various physiological and pathological processes. Researchers interested in understanding the molecular mechanisms underlying red blood cell development and related disorders will find this antibody indispensable in their studies.
Product Name:
HBG1/HBG2 Polyclonal Antibody
SKU:
CAB23436
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HBG1/HBG2 (NP_000175.1).
Sequence:
MGHF TEED KATI TSLW GKVN VEDA GGET LGRL LVVY PWTQ RFFD SFGN LSSA SAIM GNPK VKAH GKKV LTSL GDAI KHLD DLKG TFAQ LSEL HCDK LHVD
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Synonyms:
TNCY; HBG-T1; HBG1/HBG2
Positive Sample:
TF-1
Conjugate:
Unconjugated
Cellular Localization:
blood microparticle, cytosol, hemoglobin complex
Calculated MW:
16kDa
Observed MW:
13kDa
The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'- epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.
Purification Method:
Affinity purification
Gene ID:
3048
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of TF-1, using HBG1/HBG2 Rabbit pAb (CAB23436) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
