The HBG1 Polyclonal Antibody (CAB14989) is a valuable tool for researchers studying the HBG1 protein, which is involved in fetal hemoglobin production and regulation. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the HBG1 protein, this antibody enables precise detection and analysis in various cell types, making it an ideal choice for studies in hematology and developmental biology.
HBG1 plays a crucial role in the production of fetal hemoglobin, which is essential for oxygen transport in developing fetuses. Research on HBG1 is particularly relevant in the context of hemoglobinopathies, such as sickle cell disease and thalassemia, where understanding fetal hemoglobin regulation is key to developing novel therapies. By targeting HBG1, researchers can gain insights into the mechanisms underlying these conditions and identify potential therapeutic targets for intervention.
Product Name:
HBG1 Rabbit Polyclonal Antibody
SKU:
CAB14989
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HBG1 (NP_000550.2).
Sequence:
MGHF TEED KATI TSLW GKVN VEDA GGET LGRL LVVY PWTQ RFFD SFGN LSSA SAIM GNPK VKAH GKKV LTSL GDAT KHLD DLKG TFAQ LSEL HCDK LHVD
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
HBGA; HBGR; HBG-T2; HSGGL1; PRO2979; HBG1
Positive Sample:
LO2,K-562
Conjugate:
Unconjugated
Cellular Localization:
cytosol, hemoglobin complex
Calculated MW:
16kDa
Observed MW:
12kDa
The gamma globin genes (HBG1 and HBG2) are normally expressed in the fetal liver, spleen and bone marrow. Two gamma chains together with two alpha chains constitute fetal hemoglobin (HbF) which is normally replaced by adult hemoglobin (HbA) at birth. In some beta-thalassemias and related conditions, gamma chain production continues into adulthood. The two types of gamma chains differ at residue 136 where glycine is found in the G-gamma product (HBG2) and alanine is found in the A-gamma product (HBG1). The former is predominant at birth. The order of the genes in the beta-globin cluster is: 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.
Purification Method:
Affinity purification
Gene ID:
3047
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using HBG1 Rabbit pAb (CAB14989) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
