The HBA2 Polyclonal Antibody (CAB8427) is a valuable tool for researchers studying the HBA2 gene, which encodes a subunit of hemoglobin A, the major oxygen-carrying protein in red blood cells. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding to the HBA2 protein, this antibody enables accurate detection and analysis in various cell types, making it ideal for studies related to hematology and blood disorders.The HBA2 gene plays a crucial role in oxygen transport and storage in the body, making it relevant for research on conditions such as anemia, sickle cell disease, and other hemoglobinopathies.
Understanding the function and regulation of the HBA2 gene can provide insights into the pathophysiology of these disorders and aid in the development of targeted treatments and therapies.Overall, the HBA2 Polyclonal Antibody (CAB8427) is a valuable tool for researchers investigating the role of the HBA2 gene in normal physiology and disease states, offering reliable and specific detection of the HBA2 protein in experimental settings.
Product Name:
HBA2 Rabbit Polyclonal Antibody
SKU:
CAB8427
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human HBA2 (NP_000508.1).
The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
Purification Method:
Affinity purification
Gene ID:
3040
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of mouse liver, using HBA2 antibody (CAB8427) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
