The HBA1 Polyclonal Antibody (CAB14551) is a valuable tool for researchers studying hemoglobin alpha 1 (HBA1), a critical component of normal hemoglobin function in red blood cells. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.HBA1 plays a vital role in oxygen transport in the body, making it a key molecule in understanding conditions like anemia and other blood disorders. By targeting HBA1 with this polyclonal antibody, researchers can accurately detect and analyze the protein in various cell types, providing valuable insights into the biology of hemoglobin and its role in health and disease.
With its high reactivity and specificity, the HBA1 Polyclonal Antibody is an essential tool for studies in hematology, transfusion medicine, and related fields. Whether investigating genetic mutations affecting hemoglobin function or exploring new therapies for blood disorders, this antibody offers reliable and accurate detection of HBA1, facilitating impactful research discoveries.
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Hemoglobin subunit alpha (Hemoglobin subunit alpha (HBA1)) (NP_000549.1).
The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
Purification Method:
Affinity purification
Gene ID:
3039
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from K-562 cells, using Hemoglobin subunit alpha (HBA1) Rabbit pAb (CAB14551) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
