The HAUS8 Polyclonal Antibody (CAB7847) is a valuable tool for researchers studying the HAUS8 protein, a key component of the Augmin complex involved in microtubule nucleation. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the HAUS8 protein, this antibody enables the detection and analysis of HAUS8 in various cell types, making it ideal for studies in cell biology, microtubule dynamics, and mitotic spindle formation.The HAUS8 protein is essential for proper mitotic spindle assembly and accurate chromosome segregation during cell division. Dysregulation of HAUS8 function has been linked to defects in cell division and chromosomal instability, which are common features of cancer cells.
Research on HAUS8 and its role in mitotic processes is crucial for understanding the mechanisms underlying tumorigenesis and identifying potential targets for cancer therapy.Overall, the HAUS8 Polyclonal Antibody is a valuable tool for researchers investigating the functions and regulatory mechanisms of the HAUS8 protein in cellular processes, particularly those related to mitosis and chromosome segregation. Its high specificity and sensitivity make it a reliable reagent for immunological assays in the fields of cell biology, cancer research, and drug discovery.
Product Name:
HAUS8 Rabbit Polyclonal Antibody
SKU:
CAB7847
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 166-410 of human HAUS8 (NP_219485.1).
Cytoplasm, centrosome, cytoskeleton, microtubule organizing center, spindle, spindle pole.
Calculated MW:
45kDa
Observed MW:
41kDa
HAUS8 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).
Purification Method:
Affinity purification
Gene ID:
93323
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HAUS8 antibody (CAB7847) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
