The HAS1 Polyclonal Antibody (CAB10453) is a valuable tool for researchers studying Hyaluronan synthase 1 (HAS1), an enzyme involved in the biosynthesis of hyaluronan, an important component of the extracellular matrix. The antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting HAS1 in human samples, making it suitable for use in Western blot applications.HAS1 plays a crucial role in regulating cell adhesion, migration, and signaling processes, making it a key player in various physiological and pathological conditions, including wound healing, inflammation, and cancer progression. By targeting HAS1, researchers can gain valuable insights into the mechanisms underlying these processes and potentially identify new therapeutic targets for diseases associated with dysregulated hyaluronan metabolism.
This antibody provides a reliable tool for investigating the expression and function of HAS1 in different cell types and experimental settings, making it an essential reagent for studies in the fields of cell biology, cancer research, and tissue regeneration. With its proven performance and versatility, the HAS1 Polyclonal Antibody (CAB10453) is a valuable asset for researchers seeking to expand our understanding of hyaluronan biology and its role in health and disease.
Product Name:
HAS1 Rabbit Polyclonal Antibody
SKU:
CAB10453
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 80-390 of human HAS1 (NP_001514.2).
Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS1 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to the hasA gene product of Streptococcus pyogenes, a glycosaminoglycan synthetase (DG42) from Xenopus laevis, and a recently described murine hyaluronan synthase. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
3036
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HAS1 antibody (CAB10453) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
