The HARS2 Polyclonal Antibody (CAB7785) is a valuable tool for researchers studying HARS2, a mitochondrial protein involved in the biosynthesis of glycine. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.HARS2 is a key player in the synthesis of mitochondrial-encoded proteins and its dysregulation has been linked to various diseases, including neurological disorders and developmental delays. By targeting the HARS2 protein, researchers can investigate its function in cellular metabolism and explore its potential role in disease pathogenesis.
With its high reactivity and specificity, the HARS2 Polyclonal Antibody is ideal for studies in biochemistry, molecular biology, and mitochondrial research. By enabling the detection and analysis of HARS2 in various cellular contexts, this antibody opens up new avenues for understanding the role of this protein in cellular physiology and disease mechanisms.
Product Name:
HARS2 Rabbit Polyclonal Antibody
SKU:
CAB7785
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 217-506 of human HARS2 (NP_036340.1).
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is an enzyme belonging to the class II family of aminoacyl-tRNA synthetases. Functioning in the synthesis of histidyl-transfer RNA, the enzyme plays an accessory role in the regulation of protein biosynthesis. The gene is located in a head-to-head orientation with HARS on chromosome five, where the homologous genes likely share a bidirectional promoter. Mutations in this gene are associated with the pathogenesis of Perrault syndrome, which involves ovarian dysgenesis and sensorineural hearing loss. Alternative splicing results in multiple transcript variants of this gene.
Purification Method:
Affinity purification
Gene ID:
23438
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HARS2 antibody (CAB7785) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
