The HARS Polyclonal Antibody (CAB7899) is a valuable tool for researchers studying Histidyl-tRNA synthetase (HARS), an enzyme involved in protein synthesis and amino acid metabolism. This antibody, produced in rabbits, has high specificity for detecting HARS in human samples and is validated for use in Western blotting experiments.HARS is essential for the accurate translation of genetic information into proteins, making it a key player in cellular function and metabolism. Dysregulation of HARS has been linked to various diseases, including neurological disorders and cancer, making it a promising target for therapeutic interventions.
By utilizing the HARS Polyclonal Antibody, researchers can explore the role of HARS in different cell types and tissues, providing insights into its function and potential implications in disease pathology. This antibody is a valuable asset for studies in biochemistry, molecular biology, and drug discovery, advancing our understanding of HARS and its significance in health and disease.
Product Name:
HARS Rabbit Polyclonal Antibody
SKU:
CAB7899
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HARS (NP_002100.2).
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3035
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HARS antibody (CAB7899) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
