The HAO1 Polyclonal Antibody (CAB6470) is a valuable tool for researchers studying the HAO1 protein, which plays a key role in the regulation of heme oxygenase activity in cells. This antibody, produced through rabbit immunization, has high reactivity with human samples and has been validated for use in Western blot applications. By targeting the HAO1 protein, this antibody enables researchers to detect and analyze its expression in various cell types, making it an essential tool for studies in biochemistry and cell biology.
HAO1 is a crucial enzyme involved in the breakdown of heme molecules, a process that is essential for maintaining cellular homeostasis and preventing oxidative stress. Dysregulation of HAO1 has been linked to various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes. Investigating the function of HAO1 with the help of this antibody can provide valuable insights into the underlying mechanisms of these diseases and may lead to the development of novel therapeutic strategies.
Product Name:
HAO1 Rabbit Polyclonal Antibody
SKU:
CAB6470
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human HAO1/GOX (NP_060015.1).
This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site.
Purification Method:
Affinity purification
Gene ID:
54363
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using HAO1/GOX Rabbit pAb (CAB6470) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
