Anti-HAND1 Antibody (CAB9855)
- SKU:
- CAB9855
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
Anti-HAND1 Antibody (CAB9855)
The Hand1 Polyclonal Antibody (CAB9855) is a essential tool for researchers studying Hand1, a transcription factor involved in heart development and cardiac morphogenesis. This polyclonal antibody was raised in rabbits and is highly specific to human samples, making it an ideal choice for Western blot applications. It binds specifically to the Hand1 protein, allowing for accurate detection and analysis in various cell types.Hand1 plays a crucial role in the development of the heart, lung, and other organs, making it a valuable target for research in cardiovascular biology and developmental biology.
Understanding the function and regulation of Hand1 is essential for uncovering its role in heart disease, developmental defects, and other related conditions. This polyclonal antibody provides researchers with a reliable tool for investigating the mechanisms underlying Hand1 activity, opening up new possibilities for therapeutic interventions in cardiovascular and developmental disorders.
| Antibody Name: | Anti-HAND1 Antibody |
| Antibody SKU: | CAB9855 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 16-215 of human HAND1 (NP_004812.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | SH-SY5Y, A-549, NIH/3T3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 16-215 of human HAND1 (NP_004812.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PHPA HPML HEPF LFGP ASRC HQER PYFQ SWLL SPAD AAPD FPAG GPPP AAAA AATA YGPD ARPG QSPG RLEA LGGR LGRR KGSG PKKE RRRT ESIN SAFA ELRE CIPN VPAD TKLS KIKT LRLA TSYI AYLM DVLA KDAQ SGDP EAFK AELK KADG GRES KRKR ELQQ HEGF PPAL GPVE KRIK GRTG WPQQ VWAL ELNQ |
| Gene ID: | 9421 |
| Uniprot: | O96004 |
| Cellular Location: | Nucleus, nucleolus, nucleoplasm |
| Calculated MW: | 23kDa |
| Observed MW: | 30kDa |
| Synonyms: | HAND1, Hxt, Thing1, bHLHa27, eHand |
| Background: | The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. |
| UniProt Protein Function: | HAND1: Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'- NRTCTG-3' (non-canonical E-box). |
| UniProt Protein Details: | Protein type:Transcription factor; Nucleolus; Cell development/differentiation; DNA-binding Chromosomal Location of Human Ortholog: 5q33 Cellular Component: cytoplasm; nucleus Molecular Function:bHLH transcription factor binding; identical protein binding; protein binding; transcription coactivator activity; transcription corepressor activity; transcription factor binding Biological Process: angiogenesis; blastocyst development; embryonic heart tube development; heart development; heart looping; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; trophectodermal cell differentiation; ventricular cardiac muscle morphogenesis |
| NCBI Summary: | The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O96004 |
| NCBI GenInfo Identifier: | 10720015 |
| NCBI Gene ID: | 9421 |
| NCBI Accession: | O96004.1 |
| UniProt Related Accession: | O96004 |
| Molecular Weight: | 23,627 Da |
| NCBI Full Name: | Heart- and neural crest derivatives-expressed protein 1 |
| NCBI Synonym Full Names: | heart and neural crest derivatives expressed 1 |
| NCBI Official Symbol: | HAND1Â Â |
| NCBI Official Synonym Symbols: | Hxt; eHand; Thing1; bHLHa27Â Â |
| NCBI Protein Information: | heart- and neural crest derivatives-expressed protein 1 |
| UniProt Protein Name: | Heart- and neural crest derivatives-expressed protein 1 |
| UniProt Synonym Protein Names: | Class A basic helix-loop-helix protein 27; bHLHa27; Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1; eHAND |
| Protein Family: | Heart- and neural crest derivatives-expressed protein |
| UniProt Gene Name: | HAND1Â Â |
| UniProt Entry Name: | HAND1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HAND1 antibody (CAB9855) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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