The HAL Polyclonal Antibody (CAB13021) is a versatile tool for research involving HAL, a key enzyme in the synthesis of histidine. This antibody, raised in rabbits, exhibits high reactivity with human samples and is suitable for Western blot applications. By binding specifically to the HAL protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential reagent for studies in biochemistry and molecular biology.HAL, also known as histidase, plays a crucial role in histidine metabolism by catalyzing the conversion of histidine to urocanic acid.
Dysregulation of HAL activity has been linked to various metabolic disorders and neurological diseases, highlighting the importance of studying this enzyme in different physiological contexts. The HAL Polyclonal Antibody offers researchers a valuable tool for investigating the function and regulation of HAL, facilitating advancements in our understanding of histidine metabolism and its implications in human health.
Product Name:
HAL Rabbit Polyclonal Antibody
SKU:
CAB13021
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human HAL (NP_002099.1).
Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3034
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using HAL Rabbit pAb (CAB13021) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
