The HADHB Polyclonal Antibody (CAB5716) is a valuable tool for researchers studying the HADHB protein, an essential enzyme involved in fatty acid beta-oxidation. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the HADHB protein, this antibody facilitates the detection and analysis of HADHB in various cell types, making it an ideal choice for studies in metabolism, lipid metabolism disorders, and mitochondrial function.HADHB plays a crucial role in breaking down fatty acids to generate energy, making it a key player in cellular metabolism.
Dysregulation of HADHB has been implicated in various metabolic disorders, including fatty acid oxidation disorders and mitochondrial dysfunction. By studying the function of HADHB, researchers can gain insight into these conditions and potentially identify new therapeutic targets for treatment.Overall, the HADHB Polyclonal Antibody (CAB5716) is a valuable tool for researchers interested in understanding the role of HADHB in metabolism and related diseases. Its specificity and reliability make it a valuable asset in the study of fatty acid beta-oxidation and mitochondrial function.
Product Name:
HADHB Rabbit Polyclonal Antibody
SKU:
CAB5716
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 34-270 of human HADHB (NP_000174.1).
This gene encodes the beta subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the beta subunit catalyzing the 3-ketoacyl-CoA thiolase activity. The encoded protein can also bind RNA and decreases the stability of some mRNAs. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. Mutations in this gene result in trifunctional protein deficiency. Alternatively spliced transcript variants encoding different isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
3032
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using HADHB Rabbit pAb (CAB5716) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
