The HADHA Polyclonal Antibody (CAB13310) is a valuable tool for researchers studying the HADHA protein, an essential enzyme involved in fatty acid beta-oxidation. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the HADHA protein, this antibody enables the detection and analysis of HADHA in various cell types, making it a valuable resource for studies in metabolism and energy production.
HADHA plays a crucial role in breaking down fatty acids to generate energy for cells, making it an important regulator of metabolic pathways. Dysregulation of HADHA has been implicated in various metabolic disorders, including fatty acid oxidation disorders and mitochondrial diseases. By understanding the function and regulation of HADHA, researchers can gain insights into the mechanisms underlying these conditions and potentially develop targeted therapies to address them.
Product Name:
HADHA Rabbit Polyclonal Antibody
SKU:
CAB13310
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 484-763 of human HADHA (NP_000173.2).
WB,1:2000 - 1:6000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
GBP; ECHA; HADH; LCEH; MTPA; LCHAD; TP-ALPHA; HA
Positive Sample:
293T,K-562
Conjugate:
Unconjugated
Cellular Localization:
Mitochondrion.
Calculated MW:
83kDa
Observed MW:
78kDa
This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation.
Purification Method:
Affinity purification
Gene ID:
3030
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from K-562 cells, using [KO Validated] HADHA Rabbit pAb (CAB13310) at 1:5000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
