HACD2 Antibody (PACO37102)
- SKU:
- PACO37102
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | HACD2 Antibody (PACO37102) |
| Antibody SKU: | PACO37102 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 protein (7-41AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot. All lanes: HACD2 antibody at 4µg/ml. Lane 1: MCF-7 whole cell lysate. Lane 2: LO2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 29 kDa. Observed band size: 29 kDa. |
 | Immunofluorescent analysis of MCF-7 cells using PACO37102 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Immunohistochemistry of paraffin-embedded human prostate cancer using PACO37102 at dilution of 1:100. |
| Background: | Catalyzes the third of the four reactions of the long-chain fatty acid, elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acid, /VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid, elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. |
| Synonyms: | Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 (EC 4.2.1.134) (3-hydroxyacyl-CoA dehydratase 2) (HACD2) (Protein-tyrosine phosphatase-like member B), HACD2, PTPLB |
| UniProt Protein Function: | PTPLB: Responsible for the dehydration step in very long-chain fatty acids (VLCFAs) synthesis. Belongs to the very long-chain fatty acids dehydratase HACD family.Protein type: Membrane protein, multi-pass; EC 4.2.1.134; Membrane protein, integral; Lipid Metabolism - unsaturated fatty acid biosynthesisChromosomal Location of Human Ortholog: 3q21.1Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membraneMolecular Function: 3-hydroxyacyl-CoA dehydratase activity; enzyme binding; protein binding; protein tyrosine phosphatase activityBiological Process: cellular lipid metabolic process; fatty acid elongation; sphingolipid biosynthetic process; triacylglycerol biosynthetic process; very-long-chain fatty acid biosynthetic process |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q6Y1H2 |
| NCBI GenInfo Identifier: | 38257153 |
| NCBI Gene ID: | 201562 |
| NCBI Accession: | NP_940684.1 |
| UniProt Secondary Accession: | Q6Y1H2 |
| UniProt Related Accession: | Q6Y1H2 |
| Molecular Weight: | 28,368 Da |
| NCBI Full Name: | very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 |
| NCBI Synonym Full Names: | 3-hydroxyacyl-CoA dehydratase 2 |
| NCBI Official Symbol: | HACD2Â Â |
| NCBI Official Synonym Symbols: | PTPLBÂ Â |
| NCBI Protein Information: | very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 |
| UniProt Protein Name: | Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 |
| UniProt Synonym Protein Names: | 3-hydroxyacyl-CoA dehydratase 2 |
| Protein Family: | |
| UniProt Gene Name: | HACD2Â Â |
| UniProt Entry Name: | HACD2_HUMAN |