The HACD2 Polyclonal Antibody (PAC037102) is a valuable tool for researchers studying the HACD2 protein, which plays a key role in lipid metabolism and fatty acid biosynthesis. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.HACD2 is involved in the elongation of long-chain fatty acids, a process essential for maintaining membrane structure and function in cells. Dysregulation of HACD2 expression has been linked to metabolic disorders, making it a potential target for therapeutic intervention in conditions such as obesity and diabetes.
By targeting the HACD2 protein, researchers can gain insights into lipid metabolism and its impact on overall cellular function. This antibody enables detection and analysis of the HACD2 protein in various cell types, providing a valuable tool for studies in metabolic pathways and potential drug development.
Western blot. All lanes: HACD2 antibody at 4µg/ml. Lane 1: MCF-7 whole cell lysate. Lane 2: LO2 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 29 kDa. Observed band size: 29 kDa.
Immunofluorescent analysis of MCF-7 cells using PACO37102 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human prostate cancer using PACO37102 at dilution of 1:100.
Background:
Catalyzes the third of the four reactions of the long-chain fatty acid, elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acid, /VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid, elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.
