The H6PD Polyclonal Antibody (CAB6440) is a valuable tool in research involving hexose-6-phosphate dehydrogenase (H6PD), an enzyme crucial for the activation of glucocorticoids in the liver and adipose tissue. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the H6PD protein, this antibody enables the detection and analysis of H6PD in various cell types, offering researchers a means to explore its role in metabolism, hormone regulation, and potential therapeutic interventions.
H6PD, also known as glucose-6-phosphate dehydrogenase, is essential for the conversion of cortisone to cortisol, a key steroid hormone involved in various physiological processes including metabolism, immune response, and stress regulation. Dysregulation of H6PD activity has been linked to metabolic disorders, inflammation, and endocrine disorders, highlighting its importance in maintaining homeostasis. Research into the function and regulation of H6PD is crucial for advancing our understanding of metabolic pathways and developing targeted therapies for related conditions.
Product Name:
H6PD Rabbit Polyclonal Antibody
SKU:
CAB6440
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human H6PD (NP_004276.2).
There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells.
Purification Method:
Affinity purification
Gene ID:
9563
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using H6PD Antibody (CAB6440) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.
