The H3F3A Polyclonal Antibody (CAB13824) is a valuable tool for researchers studying H3F3A, a histone variant involved in chromatin structure and gene regulation. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in applications such as immunofluorescence and immunohistochemistry.H3F3A plays a crucial role in epigenetic regulation, DNA repair, and maintaining genomic stability. Dysregulation of H3F3A expression has been linked to various diseases, including cancer and neurological disorders.
This antibody enables researchers to explore the function and localization of H3F3A in different cell types and tissues, providing insights into its role in disease development and progression.By utilizing the H3F3A Polyclonal Antibody, researchers can further our understanding of epigenetic mechanisms and potential therapeutic targets for treating H3F3A-related diseases. Its high specificity and versatility make it a valuable asset for studies in cancer biology, neurobiology, and epigenetics research.
Product Name:
Histone H3.3 Rabbit Polyclonal Antibody
SKU:
CAB13824
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human H3F3A (NP_002098.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Histone H3.3 Rabbit pAb (CAB13824) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.